Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system
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Influence of post-rumen supply of nutrients on rumen digesta load and voluntary intake of a roughage by sheep.
The effects of post-rumen supply of nutrients on the rumen digesta load and voluntary consumption of roughage by Border Leicester X Merino ram lambs was investigated. A chopped wheaten hay was offered to the ram lambs (n 24), whose nutrient intake was altered by infusing into the abomasum a liquid supplement, containing reconstituted cow's milk, sodium caseinate, minerals and vitamins, for a pe...
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Sodium phosphate buffer was used to extract cellulases from the plant solids fraction of rumen contents. The mixed cellulase preparation had maximal activity at pH 6.9 and 49 degrees C. The V(max) and the apparent K(m) for wheaten hay cellulose were 19.8 glucose units/min and 6.35 mg/ml, respectively, and for microcrystalline cellulose (Sigmacell) at the same enzyme concentration, they were 33 ...
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Two strictly anaerobic, crescent-shaped bacterial strains, designated WGT and Ycb08, were isolated from a cellulose-degrading mixed culture enriched from yak rumen contents. The strains were Gram-negative, non-spore-forming and motile, with four to six flagella situated at the centre of the concave side of the cell. The cells were 0.9-1.1x4-6 microm. Growth was observed at 27-46 degrees C (opti...
متن کاملSulfate reduction by a Desulfovibrio species isolated from sheep rumen.
Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism is a nonsporeforming, obligately anaerobic, mesophilic, nonmotile, gram-negative, straight rod. Cell-free extracts show absorption maxima for cytochrome c(3) and desulfoviridin, characteristic ...
متن کاملCharacterization of superoxide dismutase in the rumen bacterium Streptococcus bovis
Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron in...
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ژورنال
عنوان ژورنال: September
سال: 2019
ISSN: 2231-0916,0972-8988
DOI: 10.14202/vetworld.2019.1362-1371